Novel Cell-Based Assay to Investigate Monoacylglycerol Acyltransferase 2 Inhibitory Activity Using HIEC-6 Cell Line

The dietary triacylglycerol (TAG) gets absorbed and accumulated in the body through monoacylglycerol (MAG) pathway, which plays a major role obesity related disorders. main enzyme of this acyltransferase 2 (MGAT2), is considered as potential target for developing antiobesity compounds. Hence, there need vitro cell-based assays screening leads MGAT2 inhibitors. Because synthetic inhibitor’s side effects, an increased interest natural extracts leads. we have optimized 2-MAG-induced TAG accumulation inhibitory assay to screen using HIEC-6 cell line. A concentration-dependent was observed when cells were fed with exogenous 2-MAG. confirmed by situ BODIPY staining quantified. However, no seen DAG or TAG, suggesting MGAT2-mediated MAG uptake its conversion TAG. We demonstrated utility five different plant-based aqueous extracts. These showed various inhibition levels (25% 30%) HIEC-6. these assay. This adds new methodology screening, developing, evaluating inhibitors addressing